Polona Žnidaršič, Katarina Uršič, Aleksander Pavko
Faculty of Chemistry and Chemical Technology, University of Ljubljana,
Aškerčeva 5, SI-1000 Ljubljana, Slovenia
Studies on supporting medium composition and its feed rate were performed in order to maintain stationary biomass concentration during the process of progesterone hydroxylation with Rhizopus nigricans pellets. Growth kinetic parameters were evaluated for two media with different nitrogen sources. The results indicate the benefit of using complex media at higher feed rates than those used for the maintenance of stationary biomass concentration without exogenous steroid addition.