Jože Grdadolnik
National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
 
 

ABSTRACT
 

In this article the main advantages and limitations of the ATR* technique are presented. As examples, the ATR spectra of liquid bulk water and a water solution of protein bovine serum albumin are used. The main differences between the normal transmission spectroscopy and ATR are pointed out. Also shown is the way to calculate the optical constant, using the CIRCLE? ATR liquid cell, and from optical constants how to calculate ?' and ?’’ as well as pure absorbance spectrum, which is fully comparable with the normal transmission ones