A SQUARE WAVE VOLTAMMETRIC STUDY OF THE INTERACTION OF PROCAINE WITH DNA
Department of Chemistry,
The interaction of procaine with double-stranded DNA was studied in solution by square wave voltammetry to determine the binding constant and the binding site size using the Scatchard binding model. The binding parameters, the binding constant, K, and the effective base pair sites involved in binding, s, were obtained by the analysis of bound and free procaine concentration corresponding to the limits of slow and fast binding kinetics compared to the experimental timescale. The binding constant and the binding site size for the interaction of procaine with DNA were K = 8.78 (± 0.2) ´ 103 M-1 and s = 1.88 (± 0.06) base pairs and K = 1.60 (± 0.3) ´ 104 M-1 and s = 3.21 (± 0.04) base pairs for static and mobile binding equilibria respectively. Ionic strength and viscosity experiments suggest that procaine may bind to DNA with the groove-binding mode.
Key words: DNA, procaine, binding, voltammetry, scatchard model