SYNTHESIS AND CHEMILUMINESCENT PROPERTIES OF NOVEL BIOTINYLATED ACRIDINIUM ESTERS
and Dimitra Dimotikalib
b Chemical Engineering Department, N.T.U. Athens, Iroon
Politechniou 9, 15780
Biotinylated acridinium ester, 9-(3-biotinyloxypropyl)-10-methylacridinium-9-carboxylate trifluoromethane sulphonate, 5 and its precursors, 9-(3-hydroxypropyl)-acridine-9-carboxylate 3 and 9-(3-biotinyloxypropyl)-acridine-9-carboxylate 4 were synthesized from acridine-9-carboxylic acid and were tested for their chemiluminescent properties. In contrast to aqueous solutions, in which the chemiluminescence is very low, in polar aprotic solvents such as N,N-dimethylformamide, it increases by a few orders of magnitude. The acridinium ester 5 as well as the precursors 3 and 4 could be detected down to few picomoles.
Key words: Chemiluminescence, acridinium salts, biotin