Biodegradation of Azo Dye RO16 in Different Reactors by Immobilized Irpex lacteus

Mateja Tavčar1, Kateřina Svobodová2, Janja Kuplenk1, Čeněk Novotný2, Aleksander Pavko1

1Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000 Ljubljana, Slovenia
2Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic

Irpex lacteus is a white rot fungus known to decolorize various synthetic dyes. Decolorization of the azo dye Reactive Orange 16 by immobilized cultures of I. lacteus was compared in three different reactor systems of laboratory size: small and large trickle-bed reactors and a rotating-disc reactor. The highest dye decolorization efficiency (90% in 3 days) was observed in the small trickle bed reactor. The production of extracellular ligninolytic enzymes during dye decolorization was measured and the involvement of mycelium-associated activities in the decolorization process assessed. A repeated batch performance test demonstrated the potential of immobilized fungal cultures to decolorize synthetic dyes over long time periods.

Keywords: Irpex lacteus, dye decolorization, trickle bed reactor, rotating disc reactor, laccase, manganese-dependent peroxidase