Spectrophotometric Determination of Salicylamide and
Paracetamol in Biological Samples and Pharmaceutical Formulations by a
Differential Kinetic Method
Abbas Afkhami1, Nahid Sarlak1 and Ali Reza Zarei2
1 Department of Chemistry, Faculty of Sciences, Bu-Ali Sina University Hamadan,
Iran
2 Faculty of Materials & Chemical Engineering, Malek-Ashtar University of
Technology, Tehran, Iran
Absrtract
A rapid, simple and sensitive
spectrophotometric method is presented for the determinations of salicylamide
and paracetamol in mixture. The method is based on their complexation and
oxidation reactions by Fe3+ ion. Two sets of conditions were established such
that in one set of conditions only salicylamide reacts with Fe3+ ion, but in the
other set of conditions both of the salicylamide and paracetamol are oxidized by
Fe3+ ion in the presence of 1,10-phenantroline. In both sets the reactions can
be monitored spectrophotometrically by measuring the absorbance of the produced
copmplexes at 510 nm. The data were evaluated by proportional equations. The
method allowed the determination of salicylamide and paracetamol at
concentrations between 2.0 – 300 μg mL–1 and 0.50 - 10 μg mL–1 with relative
standard deviations of 2.58% and 3.47%, respectively. The method was applied to
the determination of salicylamide and paracetamol in human serum and
pharmaceutical formulations.
Key words: salicylamide, paracetamol