Affinity Ranking of Phage-Displayed Peptides: Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance

Mojca Lunder¹*, Tomaž Bratkovič¹, Gregor Anderluh², Borut Štrukelj¹ and Samo Kreft¹

¹Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana, Slovenia
²Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve~na pot 111, SI-1000 Ljubljana, Slovenia
* Corresponding author: E-mail: mojca.lunder@ffa.uni-lj.si;
Tel.:+38614769500; Fax: +38614258031

Abstract
Peptides with particular affinity and specificity for variety of targets are selected through panning procedure from random peptide phage display libraries. Efficiency and convenience of enzyme linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) for screening and evaluating peptide-displaying phage clones were compared using streptavidin as a model protein target.

Keywords: ELISA; surface plasmon resonance; Biacore; peptide phage display