Polypyrrole Layers Electrodeposited from TRIS Buffer Solution as Matrix of Tyrosinase Biosensor

Juliusz Adamski* and Jolanta Kochana

Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-060 Cracow, Ingardena 3, Poland
* Corresponding author: E-mail: adamskij@chemia.uj.edu.pl

A novel supporting electrolyte tris(hydroxymethyl)aminomethane hydrochloride (TRIS) buffer solution was used to obtain polypyrrole (PPy) layers based on the anodic oxidation of pyrrole. Influence of some parameters: temperature, monomer and supporting electrolyte concentration, polarization potential and hydrodynamic conditions on electrodeposition process were investigated. Obtained layers were preliminary tested in construction of an amperometric biosensor based on enzyme tyrosinase, which response was tested using catechol as a substrate.

Keywords: Biosensor, tyrosinase, polypyrrole, electropolymerization, catechol determination