Interactive Effects of Insulin and β-Estradiol on Protein Kinase B Phosphorylation in Adult Rat Cardiomyocytes

Emina Sudar,1 Branislava Dobutovic,1 Tijana Milosavljevic,1 Biljana Putnikovic,2 Zoran Gluvic2 and Esma R. Isenovic1

1 Institute Vinca, Laboratory of Radiobiology and Molecular Genetics, P.O. Box 522, 11001 Belgrade, Serbia
2 Clinical Hospital Center Zemun, Department of Endocrinology and Cardiology, Vukova 8, 11080 Zemun, Serbia

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Insulin (INS) and β-estradiol (E2) have vascular effects partially through stimulation of protein kinase B (Akt) phosphorylation. Employing adult rat cardiomyocytes (ARC), the effects of acute (15 min) stimulation with INS (10 mIU/ml) and prolonged (2 hours and 24 hours) stimulation with E2 alone or in combination, were assessed with respect to protein levels of Akt. Exposure to INS for 15 minutes enhanced Akt Ser473 phosphorylation. The combined treatment had a greater effect on Akt phosphorylation then the effect of INS alone. The results suggest that INS and E2 may interact through Akt in regulating metabolic processes.

Keywords: Adult rat cardiomyocytes, insulin, estradiol, signaling pathways, protein kinase B