Biodegradation of Navy Blue-3G by Brevibacillus laterosporus MTCC 2298
Sushama S. Gomare,1,* Satish D. Kalme2 and Sanjay P. Govindwar1
1 Department of Biochemistry, Shivaji University, Kolhapur-416004,
India
2 Department of Chemistry, College of Natural Sciences, Hanyang
University, Seoul-133-791, South Korea.
* Corresponding author: E-mail:
sushamabiochemist@gmail.com;
Tel: +91-231-2609152, Fax: +91-231-2691533
Abstract
Attempts have been made to decolorize widely used disperse azo dye Navy Blue-3G
by the Brevibacillus laterosporus MTCC 2298. The 80% decolorization was
observed within 48 h under static condition at the concentration 50 mg/L. No
significant change in the decolorization performance was observed under shaking
condition. Decolorization performance was steeply decreased at the higher
concentration (1.0 g/L) even after extended incubation (66% respectively within
72 h). Maximum decolorization exhibited at 30 °C and in broad range of pH (i. e.
7.0–11.0). Complete inhibition of tyrosinase, no significant change in the
activities of laccase and DCIP reductase where as significant increase in the
activities of lignin peroxidase (54%), aminopyrine N-demethylase (122%) and MG
reductase (50%) was found in the cells obtained after decolorization when
compared to control cells (i. e. without dye addition). Biodegradation of dye
was confirmed by the TLC (thin layer chromatography) and FTIR (Fourier Transform
Infra Red) spectroscopy. Bromobenzene (m/z 156, RT 19.892), ester substituted
aniline derivative (m/z 178, RT 20.492), meta-di nitro benzene (m/z 167,
RT 21.550) and ortho benzene aniline derivative (m/z 169, RT 23.892) were
the degradation products elected by GC-MS (Gas Chromatography-Mass Spectroscopy)
analysis. Extracted degradation products were found to be non-toxic for three
bacteria such as Brevibacillus laterosporus, Pseudomonas aeruginosa and
Azotobacter vinelandii as well as for the two crops Sorghum vulgare and
Phaseolus mungo.
Keywords: Brevibacillus laterosporus, Navy Blue-3G, Biodegradation, FTIR spectroscopy, GC-MS spectral analysis