DETERMINATION OF ACETATE IN PHARMACEUTICAL PRODUCTS BY HPLC^†

 

Svjetlana Mihaljica, Jelena Trbojević

 

Institute of Pharmacy of Serbia, Vojvode Stepe 458, Belgrade, Yugoslavia

 

Marija Mašković

 

Institute of Pharmacy of Serbia, Vojvode Stepe 458, Belgrade, Yugoslavia

 

Abstract

HPLC method, using anion exchange column and UV/Vis detector, for identification and determination of acetate in medicinal products is described in this study. SUPELCOSIL SAX1 strong anion exchange column has ion exchange character. In the ion exchange mode, different pH and ion strength will influence the retention time of acetate. Increasing the ionic strength will reduce its retention time. Changing the pH will control the protonation state of the analyte.

Acetate has been found in those products as salt L-Lysinacetate. Validation of the method establishes that its performance characteristics (figures of merits, quality of parameters) are adequate for the intended use. It entails the evaluation of number of parameters, such as selectivity, linearity, accuracy, specificity, precision (repetability and reproducibility), sensitivity, detection and determination limits and robustness.