A
SQUARE WAVE VOLTAMMETRIC STUDY OF THE INTERACTION OF PROCAINE WITH DNA
Mehmet Aslanoglu
Department of Chemistry,
Abstract
The
interaction of procaine with double-stranded DNA was studied in solution by
square wave voltammetry to determine the binding
constant and the binding site size using the Scatchard
binding model. The binding parameters, the binding constant, K, and the
effective base pair sites involved in binding, s, were obtained by the analysis
of bound and free procaine concentration corresponding to the limits of slow
and fast binding kinetics compared to the experimental timescale. The binding
constant and the binding site size for the interaction of procaine with DNA
were K = 8.78 (± 0.2) ´ 103 M-1 and s
= 1.88 (± 0.06) base pairs and K = 1.60 (± 0.3) ´ 104 M-1 and s
= 3.21 (± 0.04) base pairs for static and
mobile binding equilibria respectively. Ionic
strength and viscosity experiments suggest that procaine may bind to DNA with
the groove-binding mode.
Key words: DNA, procaine, binding, voltammetry,
scatchard model