Visualization of Amphetamine and Its Analogues in TLC

A. Zakrzewska,a,* A. Parczewski,a,b D. Kaz´mierczak,c W. Ciesielski,c J. Kochanaa

a Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-060 Cracow, Ingardena 3, Poland.
Tel.: +48(12)6632014,
Fax: +48(12)6340515,

Institute of Forensic Research, 31-033 Cracow, Westerplatte 9, Poland
Department of Instrumental Analysis, University of Łódź, 90-236 Łódź, Pomorska 163, Poland

Paper based on a presentation at the 12th International Symposium on Separation Sciences, Lipica, Slovenia,
September 27–29, 2006.

Derivatisation followed by iodine azide reaction was employed for detection of amphetamines and its analogues in TLC. The derivatisation reaction with phenyl isothiocyanate took place directly on the TLC plate before the developing step. Afterwards, the plate was sprayed with a mixture of sodium azide and starch solution and then exposed to iodine vapour. The obtained limits of detection were compared with other commonly visualization techniques: UV, iodine vapour, Marquis and Simon’s reagents, ninhydrin, Fast Black K.

Keywords: TLC, iodine azide reaction, visualization of amphetamines;