A. Zakrzewska,a,* A. Parczewski,a,b D. Kaz´mierczak,c W. Ciesielski,c J. Kochanaa
a
Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian
University, 30-060 Cracow, Ingardena 3, Poland.
Tel.: +48(12)6632014,
Fax: +48(12)6340515,
E-mail: zakrzews@chemia.uj.edu.pl.
b
Institute of Forensic Research, 31-033 Cracow, Westerplatte 9, Poland
c
Department of Instrumental Analysis, University of Łódź, 90-236 Łódź, Pomorska
163, Poland
Paper based on a
presentation at the 12th International Symposium on Separation
Sciences, Lipica, Slovenia,
September 27–29, 2006.
Abstract
Derivatisation
followed by iodine azide reaction was employed for detection of amphetamines and
its analogues in
TLC. The
derivatisation reaction with phenyl isothiocyanate took place directly on the
TLC plate before the developing step. Afterwards, the plate was sprayed with a
mixture of sodium azide and starch solution and then exposed to iodine vapour.
The obtained limits of detection were compared with other commonly visualization
techniques: UV, iodine vapour, Marquis and Simon’s reagents, ninhydrin, Fast
Black K.
Keywords: TLC, iodine azide reaction, visualization of amphetamines;