Planar Chromatography of SteroidHormones and Anabolics

Meliha Lekić,a* Fehim Korać,a Miroslav Šober,b Aleksandra Marjanovićb

a University of Sarajevo, Faculty of Medicine, Sarajevo, Bosnia and Herzegovina.
b University of Sarajevo, Faculty of Pharmacy, Sarajevo, Bosnia and Herzegovina

Paper based on a presentation at the 12th International Symposium on Separation Sciences, Lipica, Slovenia,
September 27–29, 2006.

Simultaneous separation of eleven steroid hormones and synthetic anabolics: progesterone, trenbolone acetate, melengestrol acetate, 17-ß-estradiol, 19-nortestosterone, fluoxymesterone, norethandrolone, 4-chloro-
δ-1-methyl testosterone, clostebol acetate, 6-ß-hydroxymethandienone and oxymetholone, was performed on HPTLC plates, 10 × 10 cm, silicagel 60 F254 (Merck) by horizontal elution in chloroform-acetone mobile phase. The investigated steroids were successfully visualised under UV light (254 nm), and after spraying with an ethanolic solution of p-toluenesulphonic acid. The efficacy of chromatographic system was checked using simulated real samples for some of the examined steroids, melengestrol acetate and trenbolone acetate, usually misused as growth promoters in cattle and stored unchanged in animal tissue.

Keywords: steroid hormones, steroid anabolics, HPTLC