Meliha Lekić,a* Fehim Korać,a Miroslav Šober,b Aleksandra Marjanovićb
a
University of Sarajevo, Faculty of Medicine, Sarajevo, Bosnia and Herzegovina.
E-mail: melihalekic@gmail.com.
b
University of Sarajevo, Faculty of Pharmacy, Sarajevo, Bosnia and Herzegovina
Paper based on a
presentation at the 12th International Symposium on Separation Sciences, Lipica,
Slovenia,
September 27–29, 2006.
Abstract
Simultaneous separation of
eleven steroid hormones and synthetic anabolics: progesterone, trenbolone
acetate, melengestrol acetate, 17-ß-estradiol, 19-nortestosterone,
fluoxymesterone, norethandrolone, 4-chloro-
δ-1-methyl
testosterone, clostebol acetate, 6-ß-hydroxymethandienone and
oxymetholone, was performed on HPTLC plates, 10 × 10 cm, silicagel 60 F254
(Merck) by horizontal elution in chloroform-acetone mobile phase. The
investigated steroids were successfully visualised under UV light (254 nm), and
after spraying with an ethanolic solution of p-toluenesulphonic acid. The
efficacy of chromatographic system was checked using simulated real samples for
some of the examined steroids, melengestrol acetate and trenbolone acetate,
usually misused as growth promoters in cattle and stored unchanged in animal
tissue.
Keywords: steroid hormones, steroid anabolics, HPTLC