Affinity Ranking of Phage-Displayed Peptides: Enzyme-Linked Immunosorbent Assay versus Surface Plasmon Resonance
Mojca Lunder1*, Tomaž Bratkovič1, Gregor Anderluh2, Borut Štrukelj1 and Samo Kreft1
1Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana,
2Department of Biology, Biotechnical Faculty, University of Ljubljana, Ve~na pot 111, SI-1000 Ljubljana, Slovenia
* Corresponding author: E-mail: firstname.lastname@example.org;
Tel.:+38614769500; Fax: +38614258031
Peptides with particular affinity and specificity for variety of targets are selected through panning procedure from random peptide phage display libraries. Efficiency and convenience of enzyme linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) for screening and evaluating peptide-displaying phage clones were compared using streptavidin as a model protein target.
Keywords: ELISA; surface plasmon resonance; Biacore; peptide phage display