Synthesis and Cyclization Reactions with Pyrazolopyrimidinyl Keto-esters and their Enzymaic Activity

Mohamed Abdel Hamid Abd el Gawaad Awas*

Department of Chemistry, Faculty of Education, Ain-Shams University Roxy, 11711 Cairo, Egypt
* Corresponding author: E-mail: dr.mohamedawas1@hotmail.com

Abstract
Ethyl 2,4-dioxo-4-(4-oxo-1-phenyl-1,4-dihydo-5H-pyrazolo[3,4-d]pyrimidin-5-yl)butanoate, ethyl 5-(4-oxo-1-phenyl- 1,4-dihydro-5H-pyrazolo[3,4-d]pyrimidin-5-yl)-1H-pyrazole-3-carboxylate and their acid hydrazide derivatives have been prepared and reacted with hydrazines, ortho-phenylenediamine, triethyl orthoformate, carbon disulfide and thiosemicarbazides in order to obtain some new 5-substituted pyrazolopyrimidin-4-ones as pyrazolines, isoxazolines, imidazoles, pyrazolotriazines, thiadiazoles and triazoles. All newly prepared compounds revealed the potent effect on increasing reactivity of cellobiase. Structures of new compounds were established upon their elemental analysis, IR, ¹H NMR and mass fragmentation spectra.

Keywords: Pyrazolines, isoxazolines, imidazoles, pyrazolotriazines, thiadiazoles and triazoles