Validation of Analytical Procedure for Simultaneous Determination of Two Avermectins in Various Soils

Tina Virant Celestina* and Nevenka Kožuh Eržen

Institute of Pathology, Forensic and Administrative Veterinary Medicine, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, SI-1000 Ljubljana, Slovenia
* Corresponding author: tina.virant.celestina@vf.uni-lj.si
Telephone: +386 1 4779136; Fax: +386 1 2832243

Abstract
An analytical method for determination of abamectin and/or doramectin in various soils has been developed. Classical extraction procedure for extraction of both avermectins from soils, clean-up of extracts using an appropriate solid-phase extraction (SPE) and a high performance liquid chromatography (HPLC) with fluorimetric detection (FLD) were introduced in the analytical procedure. The recoveries were in the range from 64 to 81% for abamectin and from 73 to 89% for doramectin in silty clay soils and 55 to 59% for abamectin and 63 to 70% for doramectin in clay soil, respectively. Limits of detection were 0.5 and 0.7 ng g–1 of moist soil for abamectin and doramectin, respectively for silty clay soil and 2.0 ng g^–1 for clay soil.

Keywords: Avermectins, abamectin, doramectin, analytical procedure, soil