Validation of Analytical Procedure for Simultaneous Determination of Two Avermectins in Various Soils

Tina Virant Celestina* and Nevenka Kožuh Eržen

Institute of Pathology, Forensic and Administrative Veterinary Medicine, Veterinary Faculty, University of Ljubljana, Gerbičeva 60, SI-1000 Ljubljana, Slovenia
* Corresponding author:
Telephone: +386 1 4779136; Fax: +386 1 2832243

An analytical method for determination of abamectin and/or doramectin in various soils has been developed. Classical extraction procedure for extraction of both avermectins from soils, clean-up of extracts using an appropriate solid-phase extraction (SPE) and a high performance liquid chromatography (HPLC) with fluorimetric detection (FLD) were introduced in the analytical procedure. The recoveries were in the range from 64 to 81% for abamectin and from 73 to 89% for doramectin in silty clay soils and 55 to 59% for abamectin and 63 to 70% for doramectin in clay soil, respectively. Limits of detection were 0.5 and 0.7 ng g–1 of moist soil for abamectin and doramectin, respectively for silty clay soil and 2.0 ng g–1 for clay soil.

Keywords: Avermectins, abamectin, doramectin, analytical procedure, soil